Origin
The phrase itself comes from the movie Penguins Of Madagascar that came out on November 26th, 2014. The line in the movie is said after the penguins have been captured and locked up early in the film. A clip of the scene was posted to YouTube on the channel More Android, where it received 5,000 views in one year (shown below).
The meme image of Skipper that has the text, however, isn’t the same that the scene comes from. While it is unknown which still image is used for the format, the first instance of combining the text with image was done on the upload to the /r/dankmemes subreddit by Redditor /u/Diamorphine330ml on October 22nd, 2018. The post received 54,700 upvotes and 264 comments in two years (shown below).
Source: https://knowyourmeme.com/memes/kowalski-analysis